Uji Sensitivitas Bakteri Probiotik Terhadap Vibrio harveyi Penyebab Vibriosis Secara In Vitro
Abstract
The purpose of this study was to determine the sensitivity of probiotic bacteria Bacillus sp, Photobacterium sp and Lactobacillus sp and combination of them for inhibiting the growth of V. harveyi. Sensitivity is known by measuring the diameter of the barrier formed by probiotic bacteria against V. harveyi using agar diffusion method on the Mueller Hinton Agar (MHA) medium with an incubation period of 24 hours at 300C. A seven treatment of a combination of probiotic bacteria was used. The study was arranged in a Completely Randomized Design (CRD) in 3 replications. The observational data were analyzed by analysis of variance then continued with Duncan's Real Distance Test. The results showed that the treatment for the combination of Bacillus sp with Lactobacillus sp was the best for inhibiting the growth of V.harveyi causing vibriosis.
References
Peter H. A., Nicholas S.M., Elisabeth S.M., Holt G.J., 1986. Systematic Bacteriology Vol 1. William & Wilkins. Los Angeles. 964 pp.
Gibson, G.R., Saavendra, J. M., MacFarlane, G.T., 1997. Probiotic and Intestinal Infection. In Fuller, R.(Ed.). Probiotics 2, Application and Practical Aspects. Chapman and Hall. London.
Gram, L., Melchiorsen, J., Spanggaard, B., Huber, I., Nielsen, T.F., 1999. Inhibition of Vibrio anguillarum by Pseudomonas fluorescens AH2, a Possible Probiotic Treatment of Fish. Applied and Environmental Microbiology. 65: 969.
Fooks, L.J., dan Gibson, G.R., 2002. In Vitro Investigation of The Effect of Probiotics and Prebiotics on Selected Human Intestinal Phatogens. FEMS Micobiology Ecology. 39: 67.
Irianto, A., 2003. Probiotik Akuakultur. UGM Press, Yogyakarta.
Moriarty, D. J. W., 1999. Disease Control In Shrimp Aquaculture With Probiotic Bacteria. In : Bell, C.R. ; Brylinsky, M. dan John – Green, P. (Eds). Microbial Biosystem: New Frontiers. Proceeding of The 8th International Symposium On Microbial Ecology. Atlantic Canada Society For Microbial Ecology. Halifax.
Moriarty, D. J. W., 2001. Control of Luminous Vibrio sp in Penaeid Aquaculture Ponds. Department of Chemical Engineering. The University of Quesland. Journal Aquaculture.
Sugita, H., Kawasaki, J., Kumazawa, J., Deguchi, Y., 1996. Production of Amylase by The Intestinal Bacteria of Japanese Coastal Animals. Letter In Applied Microbiology. 23: 174.
Rengpipat, S., Rukpratanporn, S., Piyatiratitivorakul, S., Menasateva, P., 2000. Immunity enhancement in Black tiger Shrimp (Penaeus monodon) by a probiont bacterium (Bacillus s11). Aquaculture : 271.
Gibson, G.R., Saavendra, J. M., MacFarlane, G.T., 1997. Probiotic and Intestinal Infection. In Fuller, R.(Ed.). Probiotics 2, Application and Practical Aspects. Chapman and Hall. London.
Gram, L., Melchiorsen, J., Spanggaard, B., Huber, I., Nielsen, T.F., 1999. Inhibition of Vibrio anguillarum by Pseudomonas fluorescens AH2, a Possible Probiotic Treatment of Fish. Applied and Environmental Microbiology. 65: 969.
Fooks, L.J., dan Gibson, G.R., 2002. In Vitro Investigation of The Effect of Probiotics and Prebiotics on Selected Human Intestinal Phatogens. FEMS Micobiology Ecology. 39: 67.
Irianto, A., 2003. Probiotik Akuakultur. UGM Press, Yogyakarta.
Moriarty, D. J. W., 1999. Disease Control In Shrimp Aquaculture With Probiotic Bacteria. In : Bell, C.R. ; Brylinsky, M. dan John – Green, P. (Eds). Microbial Biosystem: New Frontiers. Proceeding of The 8th International Symposium On Microbial Ecology. Atlantic Canada Society For Microbial Ecology. Halifax.
Moriarty, D. J. W., 2001. Control of Luminous Vibrio sp in Penaeid Aquaculture Ponds. Department of Chemical Engineering. The University of Quesland. Journal Aquaculture.
Sugita, H., Kawasaki, J., Kumazawa, J., Deguchi, Y., 1996. Production of Amylase by The Intestinal Bacteria of Japanese Coastal Animals. Letter In Applied Microbiology. 23: 174.
Rengpipat, S., Rukpratanporn, S., Piyatiratitivorakul, S., Menasateva, P., 2000. Immunity enhancement in Black tiger Shrimp (Penaeus monodon) by a probiont bacterium (Bacillus s11). Aquaculture : 271.
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